Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: The IL1β-IL1R signaling is involved in the stimulatory effects triggered by hypoxia in breast cancer cells and cancer-associated fibroblasts (CAFs)

doi: 10.1186/s13046-020-01667-y

Figure Lengend Snippet: a Scatter plots depicting the correlation between HIF-1α expression and IL-1β levels in TNBC patients of the TCGA and METABRIC cohorts. The Pearson correlation coefficients (r) and the relative p-values are shown in each panel. b Box plots showing the differential IL-1β expression levels in non-TNBC and TNBC patients, as found in the TCGA and METABRIC datasets. The number of patients and p -values are reported in each panel

Article Snippet: The Pearson correlation coefficients (r-values) between the mRNA levels of HIF-1α and the other genes of the TCGA (n. 20,530) dataset in TNBC cohort of patients were assessed in R Studio (version 3.6.1) using the cor.test() function and setting the method as “Pearson”.

Techniques: Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: The IL1β-IL1R signaling is involved in the stimulatory effects triggered by hypoxia in breast cancer cells and cancer-associated fibroblasts (CAFs)

doi: 10.1186/s13046-020-01667-y

Figure Lengend Snippet: a Venn diagram of metastatic genes up-regulated in MDA-MB-231 cells in an exclusive and shared manner upon hypoxia (2% O2) and IL-1β. The genes up-regulated by both hypoxia and IL-1β are listed in the box according to the gene expression changes induced by hypoxia (from high to low) (see Additional file ). b mRNA expression of IL-1β and COX2 in MDA-MB-231 cells cultured upon normoxia and hypoxia or vehicle and IL-1β in the presence or absence of the IL1R1 antagonist IL1R1a, as evaluated by real-time PCR. Values are normalized to the actin beta (ACTB) expression and shown as fold changes of the mRNA expression induced by hypoxia respect to normoxic cells. c Scatter plot depicting the correlation between the expression of COX2 and IL-1β in TNBC patients of the METABRIC cohort of patients. The Pearson correlation coefficient (r) and the relative p -value are indicated. d Immunoblots of IL-1β and COX2 in MDA-MB-231 cells cultured upon normoxia or hypoxia in combination with IL1R1a, as indicated. e Immunoblots of IL-1β and COX2 in MDA-MB-231 cells treated with vehicle or IL-1β and in combination with IL1R1a. f Immunoblots of COX2 in MDA-MB-231 cells upon normoxia and hypoxia silencing GPER. Side panels show densitometric analysis of the blots normalized to β-actin. Values represent the mean ± SD of three independent experiments performed in triplicate. (*) p < 0.05 for cells cultured under normoxia versus cells cultured under hypoxia or cells treated with IL-1β versus vehicle-treated cells

Article Snippet: The Pearson correlation coefficients (r-values) between the mRNA levels of HIF-1α and the other genes of the TCGA (n. 20,530) dataset in TNBC cohort of patients were assessed in R Studio (version 3.6.1) using the cor.test() function and setting the method as “Pearson”.

Techniques: Gene Expression, Expressing, Cell Culture, Real-time Polymerase Chain Reaction, Western Blot